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Product Description
Human Parathyroid Hormone Related Protein (PTHrP) ELISA Kit | KTE61063 | Abbkine
Application: This Human Parathyroid Hormone Related Protein (PTHrP) ELISA Kit employs a two-site sandwich ELISA to quantitate PTHLH in samples. An antibody specific for PTHLH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTHLH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTHLH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTHLH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Parathyroid Hormone Related Protein microplate
• Human Parathyroid Hormone Related Protein standard
• Human Parathyroid Hormone Related Protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Parathyroid Hormone Related Protein (PTHrP) ELISA Kit has high sensitivity and excellent specificity for detection of Human PTHLH. No significant cross-reactivity or interference between Human PTHLH and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Parathyroid hormone-related protein (or PTHrP) is a protein occasionally secreted by cancer cells (breast cancer, certain types of lung cancer including squamous cell carcinoma) . However, it also has normal functions. PTHrP acts as an endocrine, autocrine/ paracrine, and intracrine hormone. PTHrP is related in function to the "normal" parathyroid hormone. When a tumor secretes PTHrP, this can lead to hypercalcemia. As this is sometimes the first sign of the malignancy, hypercalcemia caused by PTHrP is considered a paraneoplastic phenomenon. PTHrP shares the same N-terminal end as parathyroid hormone and therefore it can bind to the same receptor (the Type I PTH receptor) . PTHrP aids in normal mammary gland development and lactation as well as placental transfer of calcium.
Alternative Names: PTHLH; HHM; MGC14611; PLP; PTHR; PTHRP; PTH-related protein; humoral hypercalcemia of malignancy; osteostatin; parathyroid hormone-like protein; parathyroid hormone-like related protein; parathyroid hormone-rel
Search name: PTHLH; HHM; MGC14611; PLP; PTHR; PTHRP; PTH-related protein; humoral hypercalcemia of malignancy; osteostatin; parathyroid hormone-like protein; parathyroid hormone-like related protein; parathyroid hormone-rel
Tag: PTHLH
