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Product Description
Rat Beta-2 adrenergic receptor (ADRB2) ELISA Kit | KTE100347 | Abbkine
Application: This Rat Beta-2 adrenergic receptor (ADRB2) ELISA Kit employs a two-site sandwich ELISA to quantitate ADRB2 in samples. An antibody specific for ADRB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADRB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADRB2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADRB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Rat Beta-2 adrenergic receptor microplate
• Rat Beta-2 adrenergic receptor standard
• Rat Beta-2 adrenergic receptor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Rat Beta-2 adrenergic receptor (ADRB2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat ADRB2. No significant cross-reactivity or interference between Rat ADRB2 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: ADRb2 is directly associated with one of its ultimate effectors, the class C L-type calcium channel CaV1.2. This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca (V) 1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes.
Alternative Names: ADRB2; ADRB2R; ADRBR; B2AR; BAR; BETA2AR; beta-2 adrenergic receptor; beta-2 adrenoceptor; catecholamine receptor
Search name: ADRB2; ADRB2R; ADRBR; B2AR; BAR; BETA2AR; beta-2 adrenergic receptor; beta-2 adrenoceptor; catecholamine receptor
Tag: ADRB2
