Product Description
Rat Kinesin-like protein KIF2B (KIF2B) ELISA Kit | KTE100832 | Abbkine
Application: This Rat Kinesin-like protein KIF2B (KIF2B) ELISA Kit employs a two-site sandwich ELISA to quantitate KIF2B in samples. An antibody specific for KIF2B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKIF2B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KIF2B is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KIF2B bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Rat Kinesin-like protein KIF2B microplate
• Rat Kinesin-like protein KIF2B standard
• Rat Kinesin-like protein KIF2B detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Rat Kinesin-like protein KIF2B (KIF2B) ELISA Kit has high sensitivity and excellent specificity for detection of Rat KIF2B. No significant cross-reactivity or interference between Rat KIF2B and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Kinesins are microtubule-based motor proteins involved in the transport of organelles in eukaryotic cells. They The predicted 963-amino acid protein has 63% sequence identity to the Drosophila KHC. Immunoblot analysis using antibodies against squid KHC detected a 120-kD protein in CV-1 monkey kidney epithelial cells. Immunoblot analysis using antibodies against squid KHC detected a 120-kD protein in CV-1 monkey kidney epithelial cells. Immunofluorescence studies showed that KNS1 expressed in CV-1 cells had both a diffuse distribution and a filamentous staining pattern that coaligned with microtubules but not vimentin (VIM) intermediate filaments; the KNS1 N- and C-terminal domains, but not the alpha-helical rod domain, also colocalized with microtubules.
Alternative Names: KIF2B; FLJ53902; kinesin protein
Search name: KIF2B; FLJ53902; kinesin protein
Tag: KIF2B