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Product Description
Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit | KTE100825 | Abbkine
Application: This Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit employs a two-site sandwich ELISA to quantitate NOS1 in samples. An antibody specific for NOS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNOS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NOS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NOS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Rat Neuronal Nitric Oxide Synthase microplate
• Rat Neuronal Nitric Oxide Synthase standard
• Rat Neuronal Nitric Oxide Synthase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Rat Neuronal Nitric Oxide Synthase (nNOS/NOS1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat NOS1. No significant cross-reactivity or interference between Rat NOS1 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Nitric oxide (NO) is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter; it is implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. NO is also responsible for endothelium-derived relaxing factor activity regulating blood pressure. NO mediates tumoricidal and bactericidal actions, as indicated by the fact that inhibitors of NO synthase (NOS) block these effects. Neuronal NOS and macrophage NOS are distinct isoforms.Both the neuronal and the macrophage forms are unusual among oxidative enzymes in requiring several electron donors: FAD, flavin mononucleotide (FMN), NADPH, and tetrahydrobiopterin.
Alternative Names: NOS1; IHPS1; NOS; nNOS; nitric oxide synthase 1; neuronal
Search name: NOS1; IHPS1; NOS; nNOS; nitric oxide synthase 1; neuronal
Tag: NOS1
