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Product Description
Rat Nuclear receptor corepressor 1 (NCOR1) ELISA Kit | KTE100506 | Abbkine
Application: This Rat Nuclear receptor corepressor 1 (NCOR1) ELISA Kit employs a two-site sandwich ELISA to quantitate NCOR1 in samples. An antibody specific for NCOR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNCOR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NCOR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NCOR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Rat Nuclear receptor corepressor 1 microplate
• Rat Nuclear receptor corepressor 1 standard
• Rat Nuclear receptor corepressor 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Rat Nuclear receptor corepressor 1 (NCOR1) ELISA Kit has high sensitivity and excellent specificity for detection of Rat NCOR1. No significant cross-reactivity or interference between Rat NCOR1 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: NCOR1 encodes a protein that mediates ligand-independent transcription repression of thyroid-hormone and retinoic-acid receptors by promoting chromatin condensation and preventing access of the transcription machinery. It is part of a complex which also includes histone deacetylases and transcriptional regulators similar to the yeast protein Sin3p. NCOR2 is located between the Charcot-Marie-Tooth and Smith-Magenis syndrome critical regions on chromosome 17. Alternate splicing results in multiple transcript variants. Pseudogenes of NCOR1 are found on chromosomes 17 and 20.
Alternative Names: NCOR1; KIAA1047; MGC104216; N-CoR; TRAC1; hCIT529I10; hN-CoR; thyroid hormone- and retinoic acid receptor-associated corepressor 1
Search name: NCOR1; KIAA1047; MGC104216; N-CoR; TRAC1; hCIT529I10; hN-CoR; thyroid hormone- and retinoic acid receptor-associated corepressor 1
Tag: NCOR1
