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Product Description
Rat Prokineticin-2 (PROK2) ELISA Kit | KTE100866 | Abbkine
Application: This Rat Prokineticin-2 (PROK2) ELISA Kit employs a two-site sandwich ELISA to quantitate PROK2 in samples. An antibody specific for PROK2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPROK2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PROK2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PROK2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Rat Prokineticin-2 microplate
• Rat Prokineticin-2 standard
• Rat Prokineticin-2 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Rat Prokineticin-2 (PROK2) ELISA Kit has high sensitivity and excellent specificity for detection of Rat PROK2. No significant cross-reactivity or interference between Rat PROK2 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: PROK2 gene encodes a protein expressed in the suprachiasmatic nucleus (SCN) circadian clock that may function as the output component of the circadian clock. The secreted form of the encoded protein may also serve as a chemoattractant for neuronal precursor cells in the olfactory bulb. Proteins from other vertebrates which are similar to this gene product were isolated based on homology to snake venom and secretions from frog skin, and have been shown to have diverse functions.
Alternative Names: PROK2; BV8; KAL4; MIT1; PK2; protein Bv8 homolog
Search name: PROK2; BV8; KAL4; MIT1; PK2; protein Bv8 homolog
Tag: PROK2
