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Product Description
Human Nuclear receptor coactivator 1 (NCOA1) ELISA Kit | KTE61345 | Abbkine
Application: This Human Nuclear receptor coactivator 1 (NCOA1) ELISA Kit employs a two-site sandwich ELISA to quantitate NCOA1 in samples. An antibody specific for NCOA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNCOA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NCOA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NCOA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection Method: Colorimetric
Conjugate: N/A
Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit Component: • Human Nuclear receptor coactivator 1 microplate
• Human Nuclear receptor coactivator 1 standard
• Human Nuclear receptor coactivator 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers
Features & Benefits: Human Nuclear receptor coactivator 1 (NCOA1) ELISA Kit has high sensitivity and excellent specificity for detection of Human NCOA1. No significant cross-reactivity or interference between Human NCOA1 and analogues was observed.
Calibration Range: Please inquire
Limit Of Detection: Please inquire
Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping: Gel pack with blue ice.
Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background: Nuclear receptor coactivator 1 encoded by NCOA1 acts as a transcriptional coactivator for steroid and nuclear hormone receptors. It is a member of the p160/steroid receptor coactivator (SRC) family and like other family members has histone acetyltransferase activity and contains a nuclear localization signal, as well as bHLH and PAS domains. The product of NCOA1 binds nuclear receptors directly and stimulates the transcriptional activities in a hormone-dependent fashion. Alternatively spliced transcript variants encoding different isoforms have been identified.
Alternative Names: NCOA1; F-SRC-1; KAT13A; MGC129719; MGC129720; RIP160; SRC1; bHLHe42; bHLHe74; Hin-2 protein; OTTHUMP00000155181; OTTHUMP00000155182; PAX3/NCOA1 fusion protein; steroid receptor coactivator-1
Search name: NCOA1; F-SRC-1; KAT13A; MGC129719; MGC129720; RIP160; SRC1; bHLHe42; bHLHe74; Hin-2 protein; OTTHUMP00000155181; OTTHUMP00000155182; PAX3/NCOA1 fusion protein; steroid receptor coactivator-1
Tag: NCOA1
