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Product Description
Human Anti-TNF-alpha autoantibody (TNFA-Ab) ELISA Kit | AE62583HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: TNFA-Ab
Alternative Name: N/A
Application: ELISA
Range: 15.6-1000 pg/mL
Sensitivity: 6.1 pg/mL
Intra-Assay: ≤4.3%
Inter-Assay: ≤7.5%
Recovery: 0, 97
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Competitive ELISA
Analysis Method : Qualitative
Test Principale: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TNFA-Ab. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated TNFA-Ab and incubated. The competitive inhibition reaction is launched between with HRP labeled TNFA-Ab and unlabeled TNFA-Ab with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of TNFA-Ab in the sample. The color development is stopped and the intensity of the color is measured.
Product Overview: TNFa is synthesized as a 26 kDa, type II transmembrane protein that is 233 amino acids in length. It contains a 30 amino acid (aa) cytoplasmic domain, a 26 aa transmembrane segment, and a 177 aa extracellular region. TNFa is assembled intracellularly to form a transmembrane, non-covalently-linked homotrimeric protein. The 157 aa residue soluble form of TNFa (sTNF-αis released from the C-terminus of the transmembrane protein through the activity of TNFa-converting enzyme (TACE), a membrane -bound disintegrin metalloproteinase. Rat cells known to express TNF-αinclude B cells, colonic columnar epithelial cells, NK and CD3 CD56 hepatic natural T cells, macrophages, monocytes and monocyte-derived dendritic cells, CD4 and CD8 T cells, mast cells, neutrophils, keratinocytes, plasma cells, and adipocytes.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
