Product Description
Rat Thyroglobulin IgG antibody (TAb-IgG) ELISA Kit | AE62746RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: TAb-IgG
Alternative Name: N/A
Application: ELISA
Range: 31.25-2000 pg/mL
Sensitivity: 13.5 pg/mL
Intra-Assay: ≤4.8%
Inter-Assay: ≤8.9%
Recovery: 1, 05
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Competitive ELISA
Analysis Method : Qualitative
Test Principale: This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TAb-IgG. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP) -conjugated TAb-IgG and incubated. The competitive inhibition reaction is launched between with HRP labeled TAb-IgG and unlabeled TAb-IgG with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of TAb-IgG in the sample. The color development is stopped and the intensity of the color is measured.
Product Overview: Thyroglobulin (Tg) is a 660 kDa, dimeric protein produced by and used entirely within the thyroid gland. In earlier literature, Tg was referred to as "colloid".Thyroglobulin should not be confused with Thyroxine-binding globulin, a carrier protein responsible for carrying the thyroid hormones in the blood.Tg is used by the thyroid gland to produce the thyroid hormones thyroxine (T4) and triiodothyronine (T3) . The active form of triiodothyronine, 3, 5, 3' triiodothyronine, is produced both within the thyroid gland and periphery by 5'-deiodinase (which has been referred to as tetraiodothyronine 5' deiodinase.) Thyroglobulin levels in the blood can be used as a tumor marker for certain kinds of thyroid cancer. Thyroglobulin levels in the blood can also be elevated in cases of Graves' disease.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .