Product Description
Human Anti-Glycyl tRNA Synthetase-antibody (GARS-Ab) ELISA Kit | AE62298HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: GARS-Ab
Alternative Name: CMT2D; CMT2-D; DSMAV; GlyRS; SMAD1; EJ; Charcot-Marie-Tooth Neuropathy 2D; Glycine tRNA Ligase
Application: ELISA
Range: 15.6-1000 pg/mL
Sensitivity: 6.6 pg/mL
Intra-Assay: ≤3.5%
Inter-Assay: ≤6.6%
Recovery: 0, 81
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Qualitative
Test Principale: This assay employs a two-site sandwich ELISA to quantitate GARS-Ab in samples. An antibody specific for GARS-Ab has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGARS-Ab present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GARS-Ab is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GARS-Ab bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Glycyl-tRNA synthetase isone of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha) 2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.Defects in GARS are the cause of distal spinal muscular atrophy type V (DSMA-V) . DSMA-V is an autosomal dominant distal hereditary motor neuropathy (dHMN) with a phenotype similar to CMTD2. The main characteristic that distinguishes these disorders is the less severe distal sensory involvement in DSMA-V patients.Defects in GARS are the cause of Charcot-Marie-Tooth disease type 2D (CMT2D)
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .