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Product Description
Human Anti-tissue transglutaminase IgA (IgA-tTG) ELISA Kit | AE62972HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: IgA-tTG
Alternative Name: N/A
Application: ELISA
Range: Request Information
Sensitivity: Request Information
Intra-Assay: ≤5.9%
Inter-Assay: ≤8.3%
Recovery: 0, 96
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Qualitative
Test Principale: This assay employs a two-site sandwich ELISA to quantitate IgA-tTG in samples. An antibody specific for IgA-tTG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIgA-tTG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IgA-tTG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IgA-tTG bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: N/A
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
