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Product Description
Human Cytochrome P450 2D6 (CYP2D6) ELISA Kit | AE61320HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: CYP2D6
Alternative Name: RP4-669P10.2; CPD6; CYP2D; CYP2D@; CYP2DL1; MGC120389; MGC120390; P450-DB1; P450C2D; P450DB1; cytochrome P450 2D6|cytochrome P450; subfamily IID (debrisoquine; sparteine; etc.; -metabolizing) ; polyp
Application: ELISA
Range: 0.312-20 ng/mL
Sensitivity: 0.115 ng/mL
Intra-Assay: ≤5.8%
Inter-Assay: ≤9.3%
Recovery: 1, 07
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate CYP2D6 in samples. An antibody specific for CYP2D6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCYP2D6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP2D6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP2D6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Cytochrome P450 2D6 (CYP2D6), a member of the cytochrome P450 mixed-function oxidase system, is one of the most important enzymes involved in the metabolism of xenobiotics in the body. Whilst CYP2D6 is involved in the oxidation of a wide range of substrates of all the CYPs, there is considerable variability in its expression in the liver. The gene is located near two cytochrome P450 pseudogenes on chromosome 22q13.1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. CYP2D6 activity was tested in "healthy infants receiving an oral dose (0.3 mg/kg) of dextromethorphan (DM) at 0.5, 1, 2, 4, 6, and 12 months of age. DM and its major metabolites were measured in urine. CYP2D6 genotype was determined by polymerase chain reaction-restriction fragment length polymorphism.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
