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Product Description
Human F-box/LRR-repeat protein 5 (FBXL5) ELISA Kit | AE61077HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: FBXL5
Alternative Name: FBL4; FBL5; FLR1; F-box protein FBL5
Application: ELISA
Range: 0.78-50 ng/mL
Sensitivity: 0.4 ng/mL
Intra-Assay: ≤5.7%
Inter-Assay: ≤7.7%
Recovery: 0, 95
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate FBXL5 in samples. An antibody specific for FBXL5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFBXL5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FBXL5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FBXL5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: FBXL5 encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP2-cullin-F-box), whichダϮばϮ
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
