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Product Description
Human Proprotein convertase subtilisin/kexin type 7 (PCSK7) ELISA Kit | AE60160HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: PCSK7
Alternative Name: FLJ23503; FLJ45329; LPC; PC7; PC8; SPC7; lymphoma proprotein convertase|prohormone convertase 8; subtilisin-like|prohormone convertase PC7|proprotein convertase PC7|subtilisin/kexin-like protease PC
Application: ELISA
Range: 0.625-40 ng/mL
Sensitivity: 0.285 ng/mL
Intra-Assay: ≤5.5%
Inter-Assay: ≤8.9%
Recovery: 0, 96
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PCSK7 in samples. An antibody specific for PCSK7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPCSK7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCSK7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCSK7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Proprotein convertase subtilisin/kexin type 7 is an enzyme belongs to the subtilisin-like proprotein convertase family. The members of this family are proprotein convertases that process latent precursor proteins into their biologically active products. This encoded protein is a calcium-dependent serine endoprotease. It is structurally related to its family members, PACE and PACE4. This protein is concentrated in the trans-Golgi network, associated with the membranes, and is not secreted. It can process proalbumin and is thought to be responsible for the activation of HIV envelope glycoproteins gp160 and gp140. This gene has been implicated in the transcriptional regulation of housekeeping genes. Multiple alternatively spliced transcripts are described for this gene but their full length nature is not yet known.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
