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Product Description
Human Zyxin (ZYX) ELISA Kit | AE62165HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: ZYX
Alternative Name: ESP-2; HED-2;
Application: ELISA
Range: 0.156-10 ng/mL
Sensitivity: 0.061 ng/mL
Intra-Assay: ≤5.1%
Inter-Assay: ≤9.8%
Recovery: 0, 88
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate ZYX in samples. An antibody specific for ZYX has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZYX present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZYX is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZYX bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Focal adhesions are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
