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Product Description
Mouse Fibroblast growth factor 4 (FGF4) ELISA Kit | AE63229MO | Abebio
Species Reactivity: Mouse (Mus musculus)
Abbreviation: FGF4
Alternative Name: HBGF-4; HST; HST-1; HSTF1; K-FGF; KFGF; fibroblast growth factor 4 splice isoform|heparin secretory transforming protein 1|human stomach cancer; transforming factor from FGF-related oncogene|kaposi
Application: ELISA
Range: 7.8-500 pg/mL
Sensitivity: 3.0 pg/mL
Intra-Assay: ≤5.2%
Inter-Assay: ≤9.3%
Recovery: 1, 01
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate FGF4 in samples. An antibody specific for FGF4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFGF4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Fibroblast growth factor 4 is a protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This gene was identified by its oncogenic transforming activity.This gene and FGF3, another oncogenic growth factor, are located closely on chromosome 11. Co-amplification of both genes was found in various kinds of human tumors. Studies on the mouse homolog suggested a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
