Product Description
Mouse Glucose Regulated Protein 78 (GRP78) ELISA Kit | AE58600MO | Abebio
Species Reactivity: Mouse (Mus musculus)
Abbreviation: GRP78
Alternative Name: BIP; FLJ26106; GRP78; MIF2; Heat-shock 70kD protein-5 (glucose-regulated protein; 78kD) |heat shock 70kD protein 5 (glucose-regulated protein; 78kD) |heat shock 70kDa protein 5
Application: ELISA
Range: 0.156-10 ng/mL
Sensitivity: 0.057 ng/mL
Intra-Assay: ≤5.1%
Inter-Assay: ≤7.6%
Recovery: 0, 97
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate GRP78 in samples. An antibody specific for GRP78 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGRP78 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GRP78 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GRP78 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Binding immunoglobulin protein (BiP) is a molecular chaperone that uses ATP/ADP cycling to regulate protein folding by the protein disulfide isomerase (PDI) family of proteins. It is a 78kDa glucose-regulated heat shock protein and is involved in unfolded protein response. HSPA5 is its human gene. It binds to immunoglobulin heavy chain. When the nucleotide-binding domain of GRP78 interacts with ATP, its substrate-binding domain can interact with unfolded/misfolded protein. Subsequent ATP hydrolysis acts to strengthen the interaction between GRP78 and the unfolded/misfolded protein. Under these conditions PDI can then work to promote disulfide oxidation and rearrangement until the correct protein conformation is achieved.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .