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Product Description
Mouse Receptor-type tyrosine-protein phosphatase T (PTPRT) ELISA Kit | AE25039MO | Abebio
Species Reactivity: Mouse (Mus musculus)
Abbreviation: PTPRT
Alternative Name: RP5-1121H13.2; KIAA0283; RPTPrho; receptor protein tyrosine phosphatase
Application: ELISA
Range: Request Information
Sensitivity: Request Information
Intra-Assay: ≤4.5%
Inter-Assay: ≤9.7%
Recovery: 0, 95
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PTPRT in samples. An antibody specific for PTPRT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPTPRT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTPRT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTPRT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: T is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP possesses an extracellular region, a single transmembrane region, and two tandem intracellular catalytic domains, and thus represents a receptor-type PTP. The extracellular region contains a meprin-A5 antigen-PTP (MAM) domain, Ig-like and fibronectin type III-like repeats. The protein domain structure and the expression pattern of the mouse counterpart of this PTP suggest its roles in both signal transduction and cellular adhesion in the central nervous system. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Organism species: Homo sapiens (Human)
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
