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Product Description
Mouse WAP, kazal, immunoglobulin, kunitz and NTR domain-containing protein 2 (WFIKKN2) ELISA Kit | AE11205MO | Abebio
Species Reactivity: Mouse (Mus musculus)
Abbreviation: WFIKKN2
Alternative Name: WFIKKNRP; WAP; FS; Ig; two KU and NTR module related protein|WFIKKN-related protein|WFIKKN2 protein|multivalent protease inhibitor protein
Application: ELISA
Range: 1.56-100 pg/mL
Sensitivity: 0.72 pg/mL
Intra-Assay: ≤5.3%
Inter-Assay: ≤9.0%
Recovery: 1, 05
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate WFIKKN2 in samples. An antibody specific for WFIKKN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyWFIKKN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for WFIKKN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of WFIKKN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Wolframin is a transmembrane protein.Wolframin appears to function as a cation-selective ion channel.Mutations in this gene are associated with an autosomal recessive syndrome characterized by insulin-dependent diabetes mellitus and bilateral progressive optic atrophy, usually presenting in childhood or early adult life. Diverse neurologic symptoms, including a predisposition to psychiatric illness, may also be associated with this disorder. A large number and variety of mutations in this gene, particularly in exon 8, can be associated with this syndrome. Mutations in this gene can also cause autosomal dominant deafness 6 (DFNA6) .Highly expressed in heart followed by brain, placenta, lung and pancreas. Weakly expressed in liver, kidney and skeletal muscle. Also expressed in islet and beta-cell insulinoma cell line.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
