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Product Description
Rabbit Interleukin-5 (IL5) ELISA Kit | AE63287RB | Abebio
Species Reactivity: Rabbit (Oryctolagus cuniculus)
Abbreviation: IL5
Alternative Name: EDF; IL-5; TRF; B cell differentiation factor I|T-cell replacing factor|eosinophil differentiation factor|interleukin 5|interleukin-5
Application: ELISA
Range: Request Information
Sensitivity: Request Information
Intra-Assay: ≤4.0%
Inter-Assay: ≤9.2%
Recovery: 0, 97
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate IL5 in samples. An antibody specific for IL5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL5 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL5 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: IL-5 is secreted by a restricted number of mesenchymal cell types. In its native state, mature IL-5 is synthesized as a 115 aa, highly glycosylated 22 kDa monomer that forms a 40-50 kDa disulfide-linked homodimer. Although the content of carbohydrate is high, carbohydrate is not needed for bioactivity. Monomeric IL-5 has no activity and requires a homodimer for function, which is in contrast to the receptor-related IL-3 and GM-CSF cytokines that exist only as monomers. Just as one IL-3 and GM-CSF monomer binds to one receptor, one IL-5 homodimer is able to engage only one IL-5 receptor. It has been suggested that IL-5 (as a dimmer) undergoes a general conformational change after binding to one receptor molecule and this change precludes binding to a second receptor.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
