Product Description
Rabbit Neuronal acetylcholine receptor subunit alpha-7 (CHRNA7) ELISA Kit | AE62802RB | Abebio
Species Reactivity: Rabbit (Oryctolagus cuniculus)
Abbreviation: CHRNA7
Alternative Name: CHRNA7-2; NACHRA7; a7 nicotinic acetylcholine receptor|alpha 7 neuronal nicotinic acetylcholine receptor|alpha-7 nicotinic cholinergic receptor subunit|cholinergic receptor; nicotinic; alpha polypep
Application: ELISA
Range: Request Information
Sensitivity: Request Information
Intra-Assay: ≤5.1%
Inter-Assay: ≤7.7%
Recovery: 0, 9
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate CHRNA7 in samples. An antibody specific for CHRNA7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCHRNA7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHRNA7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHRNA7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Neuronal acetylcholine receptor subunit alpha-7 forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .