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Product Description
Rat 6-phosphofructokinase, liver type (PFKL) ELISA Kit | AE27894RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: PFKL
Alternative Name: DKFZp686G1648; DKFZp686L2097; FLJ30173; FLJ40909; PFK-B; 6-phosphofructokinase; liver type|liver phosphofructokinase|liver-type 1-phosphofructokinase|phosphofructo-1-kinase isozyme B|phosphofructoki
Application: ELISA
Range: 1.56-100 ng/mL
Sensitivity: 0.67 ng/mL
Intra-Assay: ≤5.8%
Inter-Assay: ≤6.7%
Recovery: 1
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PFKL in samples. An antibody specific for PFKL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPFKL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PFKL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PFKL bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: The PFKL gene encodes the liver isoform of phosphofructokinase (PFK) (ATP:D-fructose-6-phosphate-1-phosphotransferase, EC 2.7.1.11) . PFK catalyzes the irreversible conversion of fructose-6-phosphate to fructose-1, 6-bisphosphate and is a key regulatory enzyme in glycolysis. Mammalian PFK is a tetramer made up of various combinations of 3 subunits: muscle (PFKM), liver (PFKL), and platelet (PFKP), the genes for which are located on chromosomes 12q13, 21q22, and 10p, respectively. The composition of the tetramers differs according to the tissue type. Muscle and liver PFK are a homotetramers of 4M and 4L subunits, respectively. Erythrocytes contain both L and M subunits, which randomly tetramerize to form M4, L4, M3L, M2L2, and ML3 hybrid forms of the holoenzyme.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
