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Product Description
Rat Adenylate cyclase type 10 (ADCY10) ELISA Kit | AE23900RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: ADCY10
Alternative Name: HCA2; RP1-313L4.2; SAC; SACI; Sacy; 3 ;5 -cyclic AMP synthetase|AH-related protein|ATP pyrophosphate-lyase|adenylate cyclase 10|testicular soluble adenylyl cyclase (SAC)
Application: ELISA
Range: 0.312-20 ng/mL
Sensitivity: 0.125 ng/mL
Intra-Assay: ≤5.1%
Inter-Assay: ≤11.1%
Recovery: 0, 97
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate ADCY10 in samples. An antibody specific for ADCY10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyADCY10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADCY10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADCY10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Adenylate cyclase type 10 is a protein encoded by this gene belongs to a distinct class of mammalian adenylyl cyclase that is soluble in testis but particulate in sperm and other tissues. It is unlike other mammalian adenylyl cyclases because it is insensitive to G protein or forskolin regulation and is directly regulated by bicarbonate. It is localized in the cytoplasm and to organelles and is thought to function as a general bicarbonate sensor throughout the body. It plays an important role in the generation of cAMP in spermatozoa, playing possible roles in sperm capacitation, hypermotility, and/or the acrosome reaction.Genetic variations in this gene are associated with absorptive hypercalciuria type 2. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
