Product Description
Rat Low affinity cationic amino acid transporter 2 (SLC7A2) ELISA Kit | AE18338RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: SLC7A2
Alternative Name: ATRC2; CAT2; HCAT2; amino acid transporter; cationic 2|cationic amino acid transporter; y+ system|low-affinity cationic amino acid transporter-2|solute carrier family 7; member 2
Application: ELISA
Range: 0.156-10 ng/mL
Sensitivity: 0.069 ng/mL
Intra-Assay: ≤4.9%
Inter-Assay: ≤9.2%
Recovery: 0, 97
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate SLC7A2 in samples. An antibody specific for SLC7A2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySLC7A2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC7A2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC7A2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: The gene encodes a 658-amino acid polypeptide that is 60% similar to CAT1 (ATRC1) . CAT1 and CAT2 are members of the APC family of transporters, the members of which are specific for amino acids, polyamines, and choline. Northern blot analysis revealed that CAT2 is expressed as a 9.0-kb transcript in a variety of tissues. CAT2A and CAT2B are produced by alternative splicing. The deduced CAT2A and CAT2B proteins contain 657 and 658 amino acids, respectively, and both have 14 transmembrane domains and 2 putative N-glycosylation sites in extracellular loop 3. They differ from one another in 19 residues in the vicinity of transmembrane segments 8 and 9.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .