Product Description
Rat Phospholipid scramblase 1 (PLSCR1) ELISA Kit | AE26917RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: PLSCR1
Alternative Name: MMTRA1B;
Application: ELISA
Range: 0.156-10 ng/mL
Sensitivity: 0.059 ng/mL
Intra-Assay: ≤4.1%
Inter-Assay: ≤9.4%
Recovery: 1
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PLSCR1 in samples. An antibody specific for PLSCR1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLSCR1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLSCR1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLSCR1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Scramblases are members of the general family of transmembrane lipid transporters known as flippases. The inner-leaflet facing the inside of the cell contains negatively-charged amino-phospholipids and phosphatidylethanolamine. The outer-leaflet, facing the outside environment, contains phosphatidylcholine and sphingomyelin. Scramblase is an enzyme, present in the cell membrane, that can transport (scramble) the negatively-charged phospholipids from the inner-leaflet to the outer-leaflet, and vice versa.Wiedmer et al. (2000) determined that the PLSCR1 gene contains 9 exons and spans about 30 kb. The first exon is untranslated. By deletion analysis of PLSCR1 promoter-reporter constructs, they found that 97% of the promoter activity resides between -95 bp in the 5-prime flanking region to +60 bp of the first exon.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .