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Product Description
Rat Prolargin (PRELP) ELISA Kit | AE26056RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: PRELP
Alternative Name: MGC45323; MST161; MSTP161; SLRR2A; 55 kDa leucine-rich repeat protein of articular cartilage|prolargin proteoglycan|proline arginine-rich end leucine-rich repeat protein
Application: ELISA
Range: Request Information
Sensitivity: Request Information
Intra-Assay: ≤5.3%
Inter-Assay: ≤9.9%
Recovery: 0, 85
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PRELP in samples. An antibody specific for PRELP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRELP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRELP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRELP bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: PREPL belongs to the prolyl oligopeptidase subfamily of serine peptidases. Mutations in this gene have been associated with hypotonia-cystinuria syndrome, also known as the 2p21 deletion syndrome. Several alternatively spliced transcript variants encoding either the same or different isoforms have been described for this gene. The longest deduced protein contains 727 amino acids and has a propeller domain in its N-terminal half and a catalytic domain, including the catalytic triad (ser, asp, his) essential for serine peptidases, in its C-terminal half. In sharp contrast to PREP and oligopeptidase B, which require both amino- and carboxy-terminal sequences for activity, PREPL activity appeared to depend only on the carboxy-terminal domain.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
