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Product Description
Rat Pulmonary Surfactant-associated protein D (SP-D) ELISA Kit | AE19819RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: SFTPD
Alternative Name: COLEC7; PSP-D; SFTP4; SP-D; collectin 7|pulmonary surfactant apoprotein|pulmonary surfactant-associated protein D|surfactant; pulmonary-associated protein D|surfactant-associated protein; pulmonary
Application: ELISA
Range: 7.81-500 pg/mL
Sensitivity: 3.1 pg/mL
Intra-Assay: ≤4.7%
Inter-Assay: ≤10.2%
Recovery: 0, 99
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate SFTPD in samples. An antibody specific for SFTPD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySFTPD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFTPD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFTPD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Surfactant protein D (SP-D) belongs to the collectin family.These proteins are oligomeric proteins composed of carbohydrate-recognition domains (CRD) attached to collagenous regions.Collectins are structurally similar to the ficolins although they make use of different CRD structures:C-type lectin domain for the collectins.The lung is the major site of synthesis of SP-D, where the molecules are produced and secreted onto the epithelial surface by alveolar type Ⅱcells and unciliated bronchial epithelial cells. SP-D is also found in different epithelial cells of the gastrointestinal tract and in epithelial cells of various exocrine glands. SP-D is an important factor in the pulmonary anti-microbial defense. The anti-microbial defense mechanisms of SP-D are direct opsonization, neutralization and agglutination.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
