Product Description
Rat Zona pellucida sperm-binding protein 2 (ZP2) ELISA Kit | AE62075RA | Abebio
Species Reactivity: Rat (Rattus norvegicus)
Abbreviation: ZP2
Alternative Name: ZPA; zona pellucida glycoprotein 2|zona pellucida protein A|zona pellucida sperm-binding protein 2
Application: ELISA
Range: 0.312-20 ng/mL
Sensitivity: 0.125 ng/mL
Intra-Assay: ≤6.0%
Inter-Assay: ≤7.5%
Recovery: 0, 8
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate ZP2 in samples. An antibody specific for ZP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyZP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ZP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ZP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. Zona pellucida sperm-binding protein 2 is a structural component of the zona pellucida and functions in secondary binding and penetration of acrosome-reacted spermatozoa. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .