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Product Description
Human NKG2D ligand 2 (ULBP2) ELISA Kit | AE12215HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: ULBP2
Alternative Name: N2DL2; RAET1H; ALCAN-alpha|NKG2D ligand 2|UL16-binding protein 2|retinoic acid early transcript 1 H
Application: ELISA
Range: 0.156-10 ng/mL
Sensitivity: 0.055 ng/mL
Intra-Assay: ≤4.6%
Inter-Assay: ≤8.3%
Recovery: 0, 98
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate ULBP2 in samples. An antibody specific for ULBP2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyULBP2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ULBP2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ULBP2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Human cytomegalovirus (CMV) frequently infects young people and persists for the lifetime of the individual in a latent state, due in part to downregulation of major histocompatibility (MHC) class I expression and evasion of T cell-mediated immunity, with occasional asymptomatic episodes of viral shedding. Instead of a transmembrane domain, ULBP3 has a putative glycosylphosphatidylinositol (GPI) linkage to the membrane. Enzymatic analysis confirmed that the ULBPs are indeed GPI-linked at the cell surface. RT-PCR analysis detected ULBP expression in T, B, and erythroleukemia cell lines and in a wide range of tissues. Expression was upregulated in colon and stomach tumors and downregulated in kidney tumors. FACS analysis demonstrated that protein expression generally correlated with message expression.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
