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Product Description
Human Pleckstrin homology domain-containing family O member 2 (PLEKHO2) ELISA Kit | AE26988HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: PLEKHO2
Alternative Name: DKFZp761K2312; FLJ38884; PLEKHQ1; PP1628; pp9099; PH domain-containing protein|pleckstrin homology domain containing; family Q member 1
Application: ELISA
Range: 3.125-200 pg/mL
Sensitivity: 1.25 pg/mL
Intra-Assay: ≤5.8%
Inter-Assay: ≤8.5%
Recovery: 1, 01
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PLEKHO2 in samples. An antibody specific for PLEKHO2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPLEKHO2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLEKHO2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLEKHO2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: PLEKHO2 Contains 1 PH domain.Pleckstrin homology (PH) domain family using homology based modelling as an extension of traditional sequence analysis techniques.Many of the PH domain containing proteins are found to participate in signalling cascades but there are also other classes of proteins such as cytoskeletal proteins (eg spectrin) that carry a PH domain. The PH domain family is very divergent at a sequence level, the average pairwise identity is below 20%. Despite this it is structurally very conserved with the core secondary structure superimposing well and almost all variation confined to the loops. Generall, one would need a higher identity between such small proteins to be confident about structural homology predictions and this remarkable structural conservation is one of the interesting features of this domain.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .
