Product Description
Human Poly (A) -specific ribonuclease PARN (PARN) ELISA Kit | AE28639HU | Abebio
Species Reactivity: Human (Homo sapiens)
Abbreviation: PARN
Alternative Name: DAN; deadenylating nuclease
Application: ELISA
Range: 0.781-50 ng/mL
Sensitivity: 0.27 ng/mL
Intra-Assay: ≤4.1%
Inter-Assay: ≤7.3%
Recovery: 0, 86
Sample Type: Serum, Plasma, Other biological fluids
Detection Method: Sandwich
Analysis Method : Quantitive
Test Principale: This assay employs a two-site sandwich ELISA to quantitate PARN in samples. An antibody specific for PARN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPARN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PARN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PARN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview: Poly A Specific Ribonuclease is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly (A) as the substrate, hence, efficiently degrades poly (A) tails of mRNAs. Exonucleolytic degradation of the poly (A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Poly (A) -specific ribonuclease PARN protein appears to be localized in both the nucleus and the cytoplasm. It is not stably associated with polysomes or ribosomal subunits.
Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .