Product Description
Goat anti-Dog IgG (H&L), HRP conjugated | AS16 3127 | Agrisera
Immunogen: Purified normal IgG isolated from pooled dog serum
Host: Goat
Conjugation: HRP
Clonality: Polyclonal
Isotype: N/A
Purity: Protein G purified goat IgG in PBS pH 7.2.
Format: Lyophilized
Tested Application: Dot-blot (Dot), ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Western blot (WB)
Related Products: other HRP conjugated secondary antibodies to dog IgGSecondary antibodies
Recommended Dilutions: 1 : 1000-1 : 10 000 (ELISA), 1 : 100 and 1 : 500 (ICC), (IHC), The optimal working dilution should be established by titration by the end user, Excess labelled antibody should be avoided since it may cause high unspecific background staining and interfere with the specific signal
Molecular weight: N/A
Confirmed Reactivity: Dog IgG
Predicted Reactivity: N/A
Not reactive in: This antibody does not react with any non-Ig protein in dog serum, as tested by immunoelectrophoresis and double radial immunodiffusion
Additional Information: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail. No preservative added.The reactivity of the antiserum is directed to the Fc and Fab subunits of the IgG molecule. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion.
Background: Goat anti-Dog IgG (H&L), HRP conjugated is a secondary antibody which binds to dog IgG heavy and light chain in immunological assays.
Reconstitution: For reconstitution add 1 ml of sterile destilled water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Reconstituted material can be stoored in 4°C up to 7 days.
TAIR Nnumbre: N/A
Category: HRP (horse radish peroxidase)
Research Area: Immunoassays