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Product Description
Goat anti-Human IgG Fc, HRP conjugated, min, reactivity to human IgA and IgM | AS10 664 | Agrisera
Immunogen: affinity purified human IgG Fc part
Host: Goat
Conjugation: HRP
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified goat IgG.
Format: Lyophilized
Tested Application: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Related Products: antibodies to human IgG
Recommended Dilutions: 1 : 10 000 -1 : 100 000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 10 000 -1 : 100 000 (WB)
Molecular weight: N/A
Confirmed Reactivity: Human IgG heavy chain Fc based on immunoelectrophoresis
Predicted Reactivity: Human IgG Fc
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: No reactivity to serum proteins from bovine or rabbit, No reactivity is observed to human IgA or IgM based on immunoelectrophoresis
Background: Goat anti-human IgG Fc is a secondary antibody conjugated to HRP which binds to human Fc part of IgG in immunological assays. The antibody is absorbed agains human IgA and IgM
Reconstitution: For reconstitution add 1, 1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1, 1 ml of sterile water add 1, 1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
TAIR Nnumbre: N/A
Category: IgG
Research Area: Immunoassays, Immunology
