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Product Description
Goat anti-Rabbit IgG (H&L) HRP conjugated, min, cross-reactivity to bovine/Human/mouse IgG/serum | AS10 668 | Agrisera
Immunogen: purified Rabbit IgG, whole molecule
Host: Goat
Conjugation: HRP
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified goat IgG.
Format: Lyophilized
Tested Application: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Related Products: AS09 607 | Goat anti-rabbit IgG (H&L) AP conjugatedAS09 608 | Goat anti-rabbit IgG (H&L) biotin conjugated
Recommended Dilutions: 1 : 500 -1 : 5000 (ELISA), 1 : 500 -1 : 5000 (IHC), 1 : 500 -1 : 5000 (WB)
Molecular weight: N/A
Confirmed Reactivity: heavy chains of Rabbit IgG, Light chains on all Rabbit immunoglobulins
Predicted Reactivity: Rabbit IgG Heavy and Light chains (H&L) of all Rabbit immunoglobulins
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: Based on immunoelectrophoresis no reactivity is observed to:non-immunoglobulin rabbit serum proteinsserum from bovine, human or mouseIgG from human or mouse
Background: Goat anti-rabbit IgG is a secondary antibody conjugated to HRP which binds to rabbit IgGs in immunological assays.
Reconstitution: For reconstitution add 1, 1 ml of sterile water, Let it stand 30 minutes at room temperature to dissolve, Prepare fresh working dilutions daily
Storage: Store lyophilized material at 2-8°C. For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 1 mg of antibody in 1, 1 ml of sterile water add 1, 1 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
TAIR Nnumbre: N/A
Category: HRP (horse radish peroxidase)
Research Area: Immunoassays
