Product Description
AO | ascorbate oxidase | AS09 384 | Agrisera
Immunogen: ascorbate oxidase purified from Cucurbita sp.
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Total IgG in PBS pH 7.2.
Format: Lyophilized
Tested Application: ELISA (ELISA), Immunohistochemistry (IHC), Western blot (WB)
Related Products: AS09 383 | Anti-ascrobate oxidase (AO), biotinylated rabbit antibodyPlant and algal protein extraction bufferSecondary antibodies
Recommended Dilutions: 1 : 1000-1 : 30 000 (ELISA), 1 : 1000 (WB)
Molecular weight: 61 | 45 kDa (Cucurbita pepo)
Confirmed Reactivity: Cucurbita sp.
Predicted Reactivity: Glycne max, Oryza sativa Species of your interest not listed? Contact us
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: N/A
Background: Ascorbate oxidase (AO) is an apoplastic enzyme involved in metabolism of plant ascorbate (AA) . Ascorbate (AA) plays a key role in defense against oxidative stress and is particularly abundant in photosynthetic tissues. Over 90% of the ascorbate is localized in the cytoplasm, but a substantial proportion is exported to the apoplast.
Reconstitution: For reconstitution add 0, 5 ml of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Working dilutions should be stored at 4°C, not refrozen and prefarably used the same day. If slight precipitation occurs upon storage, this should be removed by centrifugation, It will not affect the performance of the product.
TAIR Nnumbre: N/A
Category: Oxidative stress
Research Area: Plant Biology