Product Description
Delta-VPE | Vacuolar-processing enzyme delta-isozyme | AS20 4408 | Agrisera
Immunogen: Recombinant, His6-tagged, delta-VPE from Arabidopsis thaliana, UniProt: Q9LJX8, TAIR: At3g20210
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Total IgG. Protein A purified in PBS, 50% glycerol. Filter sterilized.
Format: Liquid at 2 mg/ml.
Tested Application: Immunofluorescence (IF), Immunolocalisation (IL), Western blot (WB)
Related Products: collection of antibodies to membrane transport system
Recommended Dilutions: 1: 500 (IF), 1: 5000 (IL), 1: 5000 (WB)
Molecular weight: 52 kDa (inactive precursor) | 37-38 kDa (mature, active form)
Confirmed Reactivity: Arabidopsis thaliana
Predicted Reactivity: Camelina sativa, Capsella rubella, Eutrema salsugineum, Raphanus sativus Species of your interest not listed? Contact us
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: Subcellular localisation is seed specific and restricted to developing seeds at 7 days after anthesis, Delta-VPE is detected at lower levels in flowers siliques, specifically in seed coats
Background: Delta VPE (Vacuplar-Processing Enzyme delta-isozyme) is Asparagine specific endopeptidase that may be involved in processing of proteins targeted to vacuoles (By similarity) . The enzyme is probably involved in post-translational proteolysis of seed storage proteins in the protein storage vacuole of developing seeds (PubMed:12417707, PubMed:14688293) . Exhibits a caspase-1-like activity in extracellular granules. At the early stage of seed development, required for the formation of the seed coat, by regulating cell death of specific cell layers in inner integument (Nakaune et al. 2005) .
Reconstitution: N/A
Storage: Store at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: At3g20210
Category: Vacuolar membrane
Research Area: Plant Biology