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Product Description
ELF3 | Early Flowering 3 | AS18 4168 | Agrisera
Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana ELF3, UniProt: O82804-1, TAIR: At2g25930
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified serum in PBS pH 7.4.
Format: Lyophilized
Tested Application: Chromatin immunoprecipitation (ChIP), Immunoprecipitation (IP), Western blot (WB)
Related Products: collection of antibodies to proteins involved in flowering
Recommended Dilutions: 3 µl of antibody (ChIP), 3 µl of antibody used for coating 2x20 µl Dynabeads A+G (IP), 1 : 1000 (WB)
Molecular weight: 77 kDa
Confirmed Reactivity: Arabidopsis thaliana, Brachypodium distachyon, Oryza sativa
Predicted Reactivity: Brassica rapa, Cucurbita moschata, Corchorus olitorius, Durio zibethinus, Hevea brasiliensis, Herrania umbratica, Hordeum vulgare, Manihot esculenta, Nymphaea thermarum, Olea europaea var. sylvestris, Panicum miliaceum, Phoenix dactylifera, Pisum sativum, Populus euphratica, Prunus dulcis, Ricinus communis, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Spinacia oleracea, Tanacetum cinerariifolium, Theobroma cacao, Trifolium pratense, Triticum aestivum, Zea mays, Vitis viniferaSpecies of your interest not listed? Contact us
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: ELF3 protein is extremely sensitive to light therefore extraction has to be done in green light and protein analysis has to be done using fresh extracts only. Even short light exposure cause degradation of ELF3. Extracted protein cannot be frozen but analyse directly following extraction.Antibody is confirmed to work in ChIP for Oryza sativa Nippon bare.
Background: ELF3 (Early Flowering 3) is a transcription factor, which acts within a 'zeitnehmer' feedback loop and is involved in its own circadian regulation and is a part of a corepressor complex consisting of ELF4, ELF3, and LUX involved in the transcriptional regulation of APRR9. The activity of the protein may be decreased in long day conditions due to its interaction with phytochrome B (phyB) . ELF3 is also involved in responses to nematode parasitism.Alternative names: Nematode-responsive protein
Reconstitution: For reconstitution add 50 µl, of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: At2g25930
Category: Flowering
Research Area: Plant Biology
