Product Description
PAC1 | 20S Proteasome alpha subunit C1 | AS19 4258 | Agrisera
Immunogen: Recombinant PAC1 of Arabidopsis thaliana, UniProt: O81148-1, TAIR: At3g22110, overexpressed in E.coli, purified from a gel piece
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Serum
Format: Lyophilized
Tested Application: Western blot (WB)
Related Products: AS19 4259 | Anti-PAG1 | 20S Proteasome alpha subunit G1, rabbit antibodiesAS19 4260 | Anti-PBA1 | 20S Proteasome beta subunit A1, rabbit antibodiesAS19 4261 | Anti-PBF1 | 20S proteasome beta subunit F-1, rabbit antibodiescollection of antibodies to protein modifications and enzymes involved in this process
Recommended Dilutions: 1 : 3000 (WB)
Molecular weight: 27, 4 | 26 kDa
Confirmed Reactivity: Arabidopsis thaliana
Predicted Reactivity: N/A
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: Recommended western blot conditions: SDS-PAGE, transfer to nitrocellulose, blocking 10% non-fat milk. Diluent for both primary and secondary antibodies PBS containing 0.2% Tween 20 and 1% BSA.For an image of western blot detection, refer to: Smalle et al. (2002) .
Background: PAC1 (20S Proteasome alpha subunit C1) is a component of proteasome complex which has ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The process is ATP-dependent. Alternative names: Proteasome subunit alpha type-4-A, 20S proteasome alpha subunit C-1, Proteasome 27 kDa subunit, Proteasome component 9, Proteasome subunit alpha type-3
Reconstitution: For reconstitution add 50 µl, of sterile water
Storage: Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: At3g22110
Category: Plant Proteasome
Research Area: Plant Biology