Product Description
PHOT1 | Phototropin-1 | AS10 720 | Agrisera
Immunogen: KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana PHOT1 O48963, At3g45780
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Serum
Format: Lyophilized
Tested Application: Western blot (WB)
Related Products: AS10 721 | PHOT2 | phototropin-2Plant protein extraction buffer Secondary antibodies
Recommended Dilutions: 1 : 5000 (WB)
Molecular weight: 111 | 132 kDa
Confirmed Reactivity: Arabidopsis thaliana
Predicted Reactivity: Arabidopsis thaliana
Not reactive in: Cuscuta campestris, Oryza sativa
Additional Information: Information about phot1 mutant, first named nph1: Liscum &. Briggs (1995) . Mutations in the NPH1 Locus of Arabidopsis Disrupt the Perception of Phototropic Stimuli. The Plant Cell, Vol. 7, 473-485.Huala et al. (1997) . Arabidopsis NPH1: A Protein Kinase with a Putative Redox-Sensing Domain. Science 19: Vol. 278 no. 5346 pp. 2120-2123.Lehmann et al. (2011) . Transitions of gene expression induced by short-term blue light. Plant Biology Volume 13, Issue 2, pages 349-361. Seeds of this mutant are available at uNASC.
Background: PHOT1 | phototropin-1 is a blue-light photoreceptor which containst light activated serine-threonine kinase domain. Is required for stomatal opening, chloroplast movements, leaf flattening and phototropism. Undergoes blue-light-dependent autophosphorylation. Alternative names: NPH1, RPT1, JK224, Non-phototropic hypocotyl protein 1, Root phototropism protein 1
Reconstitution: For reconstitution add 50 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: AT3G45780
Category: Photomorphogenesis
Research Area: Plant Biology