Product Description
PR-2 | GLU I | Class I beta-1, 3-glucanase | AS07 208 | Agrisera
Immunogen: Purified tobacco class I, basic ß-1, 3-glucanase. Purified GLU I consists of a mixture of closely related polypeptides encoded by a family of GLU I genes comprising GLA B5APL3 derived from the sylvestris ancestor of tobacco, GLB P27666 derived from the tomentosiformis ancestor of tobacco and homeologous recombinants (Sperisen et al., 1991) . Mature GLU I is processed from a pre-pro-polypeptide (Shinshi et al., 1988) .
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Total IgG in PBS pH 7.4. (without Ca++) .
Format: Lyophilized
Tested Application: Immunolocalization (IL), Western blot (WB)
Related Products: AS10 687 | Anti-PR-1 | Pathogenesis-related protein 1, rabbit antibodies AS12 2366 | Anti-PR-2 | pathogenesis-related protein 2, rabbit antibodies for Arabidopsis thalianaAS07 207 | Anti-PR-3 | CHN | class I chitinase, rabbit antibodiesAS12 2369 | Anti-PR-4 | Pathogenesis-related protein 4, rabbit antibodiesAS12 2373 | Anti-PR-5 | Pathogenesis-related protein 5, rabbit antibodiescollection of antibodies to other proteins involved in a response to pathogen attackSecondary antibodies
Recommended Dilutions: 8 µg/ml (WB)
Molecular weight: 37 | 33 kDa
Confirmed Reactivity: Fragaria vesca, Lactuca sativa, Nicotiana benthamiana, Nicotiana clevilandii, Nicotiana glutinosa, Nicotiana tabacum, Phalenopsis Sogo Yukidian cultivar V3, Populus sp., Solanum lycopersicum, Solanum tuberosum, Vitis vinifera
Predicted Reactivity: Dicots, Oryza sativa, Prunus persicaSpecies of your interest not listed? Contact us
Not reactive in: Arabidopsis thaliana
Additional Information: Important note: for blocking 5 % skim milk in PBS without Ca++ should be used.This antibody is purified by affinity chromarography on Portein G.
Background: Pathogenesis-related (PR) proteins, are induced in response to the infection of plants with microbial pathogens. Combinations of glucanase I and chitinase I are potent inhibitors of fungal growth in vitro however precise mechanism of that is still not known. Glucanase I and chitinase I contribute to defense against fungal infection and are currently used as markers for innate immunity, and in particular the ethylene/jasmonate signalling pathway in pathogenesis. Alternative names of the protein: basic beta-1, 3-glucanase
Reconstitution: For reconstitution add 100 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: Pathogen attack
Research Area: Plant Biology