Euro
USD
British Pound
NULL
Product Description
RPN12a | 26S proteasome regulatory subunit RPN12a | AS19 4268 | Agrisera
Immunogen: Recombinant, full length RPN12a of Arabidopsis thaliana protein sequence UniProt: Q9SGW3-1, TAIR: At1g64520, overexpressed in E.coli
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Serum
Format: Lyophilized
Tested Application: Western blot (WB)
Related Products: AS19 4260 | Anti-PBA1 | 20S Proteasome beta subunit A1, rabbit antibodiesAS19 4258 | PAC1 | 20S Proteasome alpha subunit C1AS19 4261 | Anti-PBF1 | 20S proteasome beta subunit F-1, rabbit antibodiesAS19 4259 | Anti-PAG1 | 20S Proteasome alpha subunit G1, rabbit antibodiesAS19 4262 | Anti-RPT2a | Regulatory particle triple-A ATPase subunit 2a, rabbit antibodiesAS19 4263 | Anti-RPT4a | 26S proteasome AAA-ATPase subunit RPT4a, rabbit antibodiesAS19 4264 | Anti-26S proteasome regulatory subunit RPN1a, rabbit antibodiesAS19 4265 | Anti-RPN5a | 26S proteasome regulatory subunit, putative (RPN5), rabbit antibodiesAS19 4266 | Anti-RPN10 | 26S proteasome regulatory subunit RPN10, rabbit antibodiesAS19 4267 | Anti-RPN11 | 26S proteasome regulatory subunit RPN11, rabbit antibodies
Recommended Dilutions: 1 : 3000 (WB)
Molecular weight: 30, 7 | 30 kDa
Confirmed Reactivity: Arabidopsis thaliana
Predicted Reactivity: N/A
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: Certain proportion of both wild-type transcript and protein is still produced in the rpn12a -1 mutant, see Figures 2B and 2C Smalle et al. (2002), PVDF membrane instead of nitrocellulose, and use the primary antibody at a 1:3000 dilution in 1% non-fat dry milk in PBS, performing just a 1 hour incubation at room temperature, so those adjustments to the protocol may also help reduce the background and increase signal intensity. Recommended Western blot conditions: protein load: 10-20 µg/well, membrane: PVDF membrane, blocking with 10% nonfat milk 1h/RT, antibody incubation buffer: PBS-T. Primary antibody dilution: 1: 3000 in PBS with non-fat dry milk incubation RT/1h, optimisation based on obtained result regarding signal/noise ratio.
Background: N/A
Reconstitution: For reconstitution add 50 µl, of sterile water
Storage: Store lyophilized/reconstituted at -20°C (short tem, months) or at -80°C (long term, years) ; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: At1g64520
Category: Plant Proteasome
Research Area: Plant Biology
