Product Description
Rubisco | 557 kDa hexadecamer | AS07 218 | Agrisera
Immunogen: purified 557 kDa hexadecamer Rubisco protein complex from Spinacia oleracea (SIGMA-ALDRICH R-8000), UniProt: P00875 and Q43832
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Serum
Format: Lyophilized
Tested Application: Immunolocalization (IL), Western blot (WB)
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Recommended Dilutions: 1 : 10 000-1 : 20 000 on 0, 5-10 ug total cellular protein/lane and standard (WB), 1 : 500-1:1000 (IL)
Molecular weight: 53-55 | 53-55 kDa
Confirmed Reactivity: Anabaena sp. PCC 7120, Arabidopsis thaliana, Aucuba japonica, Fremyella diplosiphon, Glycine max, Hordeum vulgare, Manihot esculenta Crantz, Oryza sativa, Physcomitrium patens, Pisum sativum, Populus sp., Salsola laricifolia, Solanum tuberosum, Spinacia oleracea, Synechocystis sp. PCC 6803, Synechococcus sp. PCC7942, Zea mays
Predicted Reactivity: higher plants, algae, Nannochloropsis sp.
Not reactive in: Chlamydomonas reinhardtii (immulolocalization)
Additional Information: RbcS subunit is not detected by this antibodyThis product can be sold containing proclin if requested
Background: Rubisco (Ribulose-1, 5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. It is demonstrably homologous from purple bacteria to flowering plants and consists of two protein subunits, each present in 8 copies. In plants and green algae, the large subunit (~55 kDa) is coded by the chloroplast rbcL gene, and the small subunit (15 kDa) is coded by a family of nuclear rbcS genes.
Reconstitution: For reconstitution add 50 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: RUBISCO/Carbon metabolism
Research Area: Plant Biology, Photosynthesis