Product Description
UGPase | UDP-glucose pyrophosphorylase (cytoplasm marker) | AS05 086 | Agrisera
Immunogen: Recombinant UGPase Q43772 overexpressed and purified from E.coli
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Serum
Format: Lyophilized
Tested Application: Immunolocalization (IL), Western blot (WB)
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Recommended Dilutions: 1 : 1500 (IL), 1 : 1000-1 : 5000 (WB)
Molecular weight: 51, 6 kDa
Confirmed Reactivity: Arabidopsis thaliana, Arabidopsis halleri, Brassica rapa, Capsicum annuum, Fortunella margarita Swingle, Citrus sinensis, Cucumis sativus, Festuca arundinacea, Hordeum vulgare, Lycopersicum esculentum, Lycopersicum chilense, Malus x domestica Borkh. c.v. Fuji, , Marchantia polymorpha, Medicago truncatula, Mesemryanthenum crystallinum, M.vaginalis, Nicotiana benthamiana, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Picea glauca, Populus sp., Solanum lycopersicum, Solanum tuberosum, Solanum sogarandinu, Triticum aestivu, Zea mays
Predicted Reactivity: Amorpha fruticosa, Bambusa oldhamii, Brachypodium distachyon, Brassica pekinensis, Capsella rubella, Cucumis melo, Eucalyptus grandis, Glycine max, Glycine soja, Gossipium hirsutum, Jatropha curcas, Pinus taeda, Populus tremula, Ricinus communis, Saccharum officinarum, Sorghum bicolor, Theobroma cacao, Zea mays, Vitis viniferaSpecies of your interest not listed? Contact us
Not reactive in: C. merolae, diatoms
Additional Information: This antibody detectes 1 ng of UGPase in a western blot and reacts with both cytosolic isoforms only which have similar MW of ca. 52 kDa in Arabidopsis thaliana.
Background: UDP-glucose pyrophosphorylase (UGPase, UDPGP) E.C=2.7.7.9. is a key enzyme of synthesis of sucrose, cellulose and other saccharides. There are two cytoplasmic isoforms of UGPase-A (which share 94 % identity on amino acid level) and one chloroplastic UGPase-B isoform in Arabidopsis thaliana which share ca. 10-11 % of identity (Kleczkowski et al. 2011) .
Reconstitution: For reconstitution add 50 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: AT3G03250
Category: Carbohydrates
Research Area: Plant Biology, Photosynthesis