Product Description
VDE | Violaxanthin de-epoxidase | AS15 3091 | Agrisera
Immunogen: His-tagged, recombinant VDE from Arabidopsis thaliana, overexpressed in E.coli, Uniprot:Q39249, TAIR: AT1G08550
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Serum
Format: Lyophilized
Tested Application: Western blot (WB)
Related Products: collection of antibodies to carotenoid metabolism
Recommended Dilutions: 1 : 2000 (WB)
Molecular weight: Propeptide 52 kDa, mature protein: 39.7 kDa, migrates at 40 kDa (Arabidopsis thaliana)
Confirmed Reactivity: Arabidopsis thaliana, Brachypodium distachyon, Deschampsia antarctica, Oryza sativa,
Predicted Reactivity: Brassica napus, Camellia sinensis, Citrus limon, Cucumis sativus, Chrysanthemum morifolium, Citrus sinensis, Coffea arabica, Fragaria ananassa, Glycine soja, Gossypium arboreum, Lactuca sativa, Lycium barbarum, Morus notabilis, Nicotiana tabacum, Phyllostachys edulis, Picea abies, Prunus humilis, Spinacia oleracea, Solanum lycopersicum, Theobroma cacao, Triticum aestivum, Zingiber officinale, Vitis viniferaSpecies of your interest not listed? Contact us
Not reactive in: diatoms
Additional Information: Extraction buffer must contain 7 M urea for Brachypodium distachyon and Oryza sativa samples.This product can be sold containing ProClin if requested.
Background: VDE (violaxanthin de-epoxidase) is a part of the xanthophyll (or violaxanthin) cycle for controlling the concentration of zeaxanthin in chloroplasts. This enzyme is catalyzing the two-step mono de-epoxidation reaction. Zeaxanthin induces the dissipation of excitation energy in the chlorophyll of the light-harvesting protein complex of photosystem II.
Reconstitution: For reconstitution add 200 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: AT1G08550
Category: Carotenoid metabolism
Research Area: Plant Biology