Product Description
Acetylated lysine (25 µl, clone 7F8) | AS10 707-25 | Agrisera
Immunogen: acetylated KLH
Host: Mouse
Conjugation: N/A
Clonality: Monoclonal
Isotype: IgG1
Purity: Total IgG fraction. Protein G purified.
Format: Liquid
Tested Application: ELISA (ELISA), Immunocytochmistry/Immunofluorescence (ICC/IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
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Recommended Dilutions: 1 : 100 (IHC), 1 : 1000 (WB)
Molecular weight: N/A
Confirmed Reactivity: Bovine, avian
Predicted Reactivity: Higher plants
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: 1 µg of this antibody is sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein and ECL detection system
Background: Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1) . The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2) . Protein posttranslational reversible lysine Nε-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3) .
Reconstitution: N/A
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: Epigenetics/DNA methylation
Research Area: Epigenetics