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Product Description
FITC | fluorescein isothiocyanate, HRP-conjugated (monoclonal, Clone NI 239) | AS18 4215 | Agrisera
Immunogen: Highly purified fluorescein isothiocyanate isomer 1,
Host: Mouse
Conjugation: HRP
Clonality: Monoclonal
Isotype: N/A
Purity: Purified IgG.1 in PBS pH 7.2, HRP conjugated.
Format: Lyophilized
Tested Application: ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (paraffin) (IHC), Dot blot (Dot), Western blot (WB)
Related Products: other tag antibodies
Recommended Dilutions: The optimum working dilution should be determined by titration by end user. 1:500 (ELISA), 1:50 to 1:200 (ICC, IHC), 1: 1000 (WB) .
Molecular weight: N/A
Confirmed Reactivity: FITC molecule
Predicted Reactivity: N/A
Not reactive in: N/A
Additional Information: This product has not been tested for cross-reactivity with other fluorescent compounds
Background: FITC is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. FITC antibody is used to enhance the specific signal from a monoclonal antibody or a secondary antibody conjugated to FITC. The sensitivity of the FITC hapten-anti-hapten system makes it a useful alternative to other systems.
Reconstitution: For reconstitution add 0, 5 ml of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube. Stort time storage at +4°C.
TAIR Nnumbre: N/A
Category: FITC/TRITC
Research Area: Tag Antibodies
