Product Description
HDEL | Endoplasmic reticulum retention signal (clone 2E7) | AS10 683 | Agrisera
Immunogen: Synthetic HDEL peptide corresponding to the C-terminus of yeast Bip protein
Host: Mouse
Conjugation: N/A
Clonality: Monoclonal
Isotype: IgG2B
Purity: Total IgG. Protein G purified in PBS pH 7.4.
Format: Liquid
Tested Application: Immunofluorescence (IF), Immunogold (IG), Western blot (WB)
Related Products: AS10 683-25 | Anti-HDEL | endoplasmic reticulum retention signal mouse antibody, 25 µgAS13 2665 | Anti-KDEL | endoplasmic reticulum retention signal (50 µg), rabbit antibodiescollection of antibodies for endomembrane proteinsPlant and algal protein extraction bufferSecondary antibodies
Recommended Dilutions: 1: 50-1 : 500 (IF), 1 : 100-1, 1000 (WB)
Molecular weight: 78 kDa
Confirmed Reactivity: Barnyard Grass (E. crus-galli), Beta vulgaris, Drosophila melanogaster, Gossypium hirsutum, Hordeum vulgare, Saccharomyces cerevisiae, Sorghum sp., Vigna radiata
Predicted Reactivity: N/A
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: Antibody in concentration 1 µg/ml was sufficient for detection of HDEL-containing proteins in 10 µg of yeast cell lysate by colorimetric western blot. Clone 2E7. For western blot results, immunofluorescence and immunogold images please referr to Napier et al. 1992.
Background: HDEL (his-asp-glu-leu) is a C-terminal tertapeptide found in yeast and plants which allows sorting of resident soluble proteins in the lumen of the endoplasmic reticulum.
Reconstitution: N/A
Storage: Store at -20°C for 1 year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: Endomembrane system
Research Area: Cell Biology