Product Description
LacI | Lactose operon repressor (clone 15C11) | AS16 3144 | Agrisera
Immunogen: Recombinant LacI-NLS-6xHis (Lactose operon repressor + C-terminal eukaryotic nuclear localization signal: SSVVHPKKKRKV (NLS) + 6xHis-tag) overexpressed in in E, coli
Host: Mouse
Conjugation: N/A
Clonality: Monoclonal
Isotype: IgG1
Purity: IgG1 immunoglobulin PBS, 0.05% (w/v) sodium azide.
Format: Liquid
Tested Application: Western blot (WB)
Related Products: Plant and algal protein extraction bufferSecondary antibodies
Recommended Dilutions: 5 µg in 10 ml (WB)
Molecular weight: 45 kDa
Confirmed Reactivity: Arabidopsis thaliana, Escherichia coli
Predicted Reactivity: Actinopolyspora erythraea, Chlamydia trachomatis, Methanosarcina mazei, Methyloglobulus morosus KOM1, Pseudomonas sp. 1-7, Shigella sonnei, Shinella sp. Species of your interest not listed? Contact us
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: N/A
Background: LacI (Lactose operon repressor) is a DNA-binding protein which represses the expression of genes encoding proteins responsible for lactose metabolism in bacteria. When lactose in absent from the bacterial medium these genes are repressed and when it becomes available it is converted to allolactose which inhibits LacI binding to DNA, thus allowing lactose metabolism.
Reconstitution: N/A
Storage: Store at -20°C, Make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: N/A
Category: Bacteria
Research Area: Bacteria