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Product Description
PsaC | PSI-C core subunit of photosystem I (PsaC antibody + PsaC protein positive control ) | AS04 042P | Agrisera
Immunogen: KLH-conjugated synthetic peptide conserved in all known PsaC proteins includingArabidopsis thaliana AtCg01060, Hordeum vulgare P69416, Oryza sativa P0C360, Chlamydomonas reinhardtii Q00914, Synechococcus elongatus Q31QV2
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Serum
Format: Lyophilized
Tested Application: Western blot (WB)
Related Products: collection of antibodies to PSI proteinsPlant and algal protein extraction buffer
Recommended Dilutions: 1 : 1000 (WB)
Molecular weight: 9 kDa
Confirmed Reactivity: Arabidopsis thaliana, Chlamydomonas reinhardtii, Cyanophora paradoxa, Heterosigma akashiwo, Emiliania huxleyi, Euglena gracilis, Gonyaulax polyedra, Horderum vulgare, Micromonas pusilla, Porphyra sp. Spinacia oleracea, Synechococcus PCC 7942, Thalassiosira pseudonan
Predicted Reactivity: Algae, Cyanobacteria, Glycine max, Nicotiana tabacum, Physcomitrium patens, Prochlorococcus sp. (surface and a deep water ecotype), Spinacia oleracea Species of your interest not listed? Contact us
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: In some species minor cross reactions with some larger proteins are seen. These may contain related iron-sulfur binding motifs. Therefore size verification of the reacting band is required. Due to the small size of the protein, care should be taken to differentiate between chemiluminescent signal from PsaC and non-specific signals from chlotophylls or lipids if pigment is retained near the bottom of the blot.Protein standard: use a load of 2 µl per well with ECL detection system and 4 µl per well with alkaline phospatase.
Background: PsaC is a conserved, chloroplast-encoded, Fe-S binding protein of approximately 10 kDa, present in all known Photosystem I complexes. It is located on the stromal side of the thylacoid membranes. PsaC coordinates the Fe-S clusters FA and FB through two cysteine-rich domains.
Reconstitution: N/A
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: ATCG01060
Category: Global Antibodies
Research Area: Plant Biology, Photosynthesis
