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Product Description
AKT1 | Potassium channel AKT1 | AS09 516 | Agrisera
Immunogen: KLH-conjugated peptide derived from Arabidopsis thaliana AKT1 Q38998, At2g26650
Host: Rabbit
Conjugation: N/A
Clonality: Polyclonal
Isotype: N/A
Purity: Immunogen affinity purified serum in PBS pH 7.4.
Format: Lyophilized
Tested Application: Western blot (WB)
Related Products: AS09 515 | Anti-KC1 | potassium channel KAT3, rabbit antibodiescollection of antibodies to membrane transport systemPlant protein extraction bufferSecondary antibodies
Recommended Dilutions: 1 : 50 with 125I (WB)
Molecular weight: 96, 9 kDa
Confirmed Reactivity: Arabidopsis thaliana
Predicted Reactivity: Arabidopsis thaliana
Not reactive in: No confirmed exceptions from predicted reactivity are currently known
Additional Information: In the work of Honsbein et al, 125I has been used for detection of KC1 since this was the only way to get enough signal after 2-phase partitioning, ECL+ has been used with the protein after expression in Sf9 insect cells (1: 1000 primary antibody dilution) and in yeast with no problem (single band detected), but these are relatively high expression systems, In native plant material ion channels are expressed in ridiculously small quantities (a few hundred proteins per cell)
Background: AKT1 is a highly selective inward-rectifying potassium channel located in cell membrane, that mediates potassium uptake by plant roots in response to low potassium conditions.
Reconstitution: For reconstitution add 100 µl of sterile water
Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
TAIR Nnumbre: AT2G26650
Category: Ion metabolism
Research Area: Metabolomics
